Ultrastructure and Chromatin Disaggregation of Human Sperm Head with Thioglycolate Treatment

The active interest in determining the ultraThe washed sperm samples were then treated with structural organization of ejaculated human sperm alkaline thioglycolate (18) . A 0 .8 M concentration of nucleus by the normal thin-sectioning technique alkaline thioglycolate was prepared by raising the has led to a state of understanding that is still pH to 9 with I N NaOH. This reagent was mixed incomplete. The findings obtained by this method 1 :1 or 1 :2 with the sperm solution . The reaction show that the nuclei of intact human sperm heads period ranged from 1 to 5 min and was followed by